Hypocreales » Cordycipitaceae » Lecanicillium

Lecanicillium magnisporum

Lecanicillium magnisporum Z.F. Zhang & L. Cai, sp. nov.

Index Fungorum number: 557634, Facesofungi number: FoF 08448; Fig. 30

Etymology: Referring to its larger conidia than other species in this genus.

Holotype: HMAS 248013.

Hyphae hyaline, septate, smooth, 0.5–2.5 μm wide. Asexual morph Conidiophores arising from aerial hyphae, erect, smooth, hyaline, 1.0–1.5 μm diam. Phialides arising from aerial hyphae solitary, or in whorls of 2–5 at the apex of conidiophores, straight or slightly curved, tapering to the apex, smooth, hyaline, 17.0–37.0 µm long, 1.0–1.5 µm diam. at base. Conidia rare, unicellular, smooth, hyaline, variable in size; macroconidia long fusiform or falcate, 9.0–16.0 × 2.0–3.0 µm (x̄ ± SD = 12.3 ± 2.0 × 2.5 ± 0.29 µm, n = 45); microconidia much fewer than macroconidia, 5.0–7.0 × 1.5–2.5 µm (x̄ ± SD = 5.94 ± 0.75 × 1.9 ± 0.41 µm, n = 20). Sexual morph not observed. Culture characteristics—Colonies on PDA attaining 44–48 mm diam. after 4 weeks, fat, felty, margin fmbriate, white. Reverse plicate, light yellow (4A2) to yellow (4A5). Colonies on OA attaining 38–40 mm diam. after 4 weeks, fat, cottony, margin entire, white. Reverse cream-white in center, pale rosybrown (6B3) at margin. Colonies on SNA attaining 45–47 mm diam. after 4 weeks, fat, tomentose, white, aerial mycelia sparse. Reverse white. Sporulation within 18 days.

 

Material examined: CHINA, Chongqing, Wulong, Erwang Cave, N 29.585°, E 108.001°, on soil, May 2016, Z.F. Zhang, HMAS 248013 (holotype designated here), ex-type living culture CGMCC3.19304 = LC12468; ibid., LC12469; ibid., LC12470; Chongqing, Wulong, Sanwang Cave, N 29.591°, E 108.001°, on soil, May 2016, Z.F. Zhang, LC12647; ibid., LC12663.

Notes: Lecanicillium magnisporum is phylogenetically allied to L. antillanum (R.F. Castañeda & G.R.W. Arnold) Zare & W. Gams, which belongs to one of the remaining clades of Lecanicillium (Fig. 25), but can be distinguished by the larger conidia (2.0–3.0 µm vs. 0.5–1.5 µm wide for marcoconidia, 5.0–7.0 × 1.5–2.5 µm vs. 2.0–3.5 × 0.5–1.5 µm for microconidia) and low sequence similarities (96% similarity, 23 bp diference in 524 bp of ITS; 99% similarity, 6 bp diference in 823 bp of LSU; 91% similarity, 73 bp diference in 820 bp of RPB2; 96% similarity, 38 bp

 

Fig. X Lecanicillium magnisporum (from ex-holotype CGMCC3.19304). a–c Upper and reverse views of cultures on PDA, OA and SNA 4 weeks after inoculation; d–f phialides and conidia; g conidiophores and phialides; h macroconidia and microconidia. Scale bars: d–h 10 μm

 

Retrieved from:

Zhang ZF, Zhou SY, Eurwilaichitr L, Ingsriswang S  et al.  2021 – Culturable mycobiota from Karst caves in China II, with descriptions of 33 new species. Fungal Diversity 106(1), 29-136. https://doi.org/10.1007/s13225-020-00453-7

 

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